The mitogenomes of two saprophytic Boletales species (Coniophora) reveals intron dynamics and accumulation of plasmid-derived and non-conserved genes

Abstract The order Boletales is a group of fungi with complex life styles, which include saprophytic and ectomycorrhizal mushroom-forming fungi. In the present study, complete mitogenomes two species, Coniophora olivacea, C. puteana, were assembled compared Boletales. Both comprised circular DNA molecules sizes 78,350 bp 79,655 bp, respectively. Comparative mitogenomic analysis indicated that species contained more plasmid-derived (7 on average) unknown functional genes (12 than four previously reported. addition, core protein coding genes, nad2 rps3, found to be subjected positive selection pressure between some species. Frequent intron gain/loss events detected in Basidiomycetes, several novel classes A total 33 introns most have undergone contraction olivacea mitogenome. Mitochondrial large-scale gene rearrangements, accumulation intra-genomic repeats mitogenome was considered as one main contributing factors. Based combined mitochondrial sets, we obtained well-supported phylogenetic tree for 76 demonstrating utility inferring Basidiomycetes phylogeny. study served first report family Coniophorineae, will help understand origin evolution patterns lifestyles.